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02:14

02:01Today we're looking at how the cell actually makes ATP.

02:06The electron transport chain pumps protons into the intermembrane space.

02:11That builds an electrochemical gradient across the inner membrane.

02:14ATP synthase then uses that gradient to phosphorylate ADP…

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Question: Why does the electron transport chain need oxygen? Answer: Oxygen is the final electron acceptor — without it, electrons back up, the proton gradient collapses, and ATP production stops. This is why aerobic respiration yields far more ATP than fermentation, which can't use the chain at all.

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Cellular Respiration — Lecture 12

Summary · Key terms · Practice questions

Key terms

ATP synthaseEnzyme that builds ATP using a proton gradient.

ChemiosmosisATP production driven by ions crossing a membrane.

Practice questions

1. How does the electron transport chain create a proton gradient?

Complexes I, III, IV pump protons into the intermembrane space as electrons pass down the chain.

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